Site identification in high-throughput RNA-protein interaction data.

ABSTRACT:

Motivation

Post-transcriptional and co-transcriptional regulation is a crucial link between genotype and phenotype. The central players are the RNA-binding proteins, and experimental technologies [such as cross-linking with immunoprecipitation- (CLIP-) and RIP-seq] for probing their activities have advanced rapidly over the course of the past decade. Statistically robust, flexible computational methods for binding site identification from high-throughput immunoprecipitation assays are largely lacking however.

Results

We introduce a method for site identification which provides four key advantages over previous methods: (i) it can be applied on all variations of CLIP and RIP-seq technologies, (ii) it accurately models the underlying read-count distributions, (iii) it allows e

SUBMITTER: Uren PJ 

PROVIDER: S-EPMC3509493 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature