Superoxide dismutase as a novel macromolecular nitric oxide carrier: preparation and characterization.

ABSTRACT: Nitric oxide (NO) is an important molecule that exerts multiple functions in biological systems. Because of the short-lived nature of NO, S-nitrosothiols (RSNOs) are believed to act as stable NO carriers. Recently, sulfhydryl (SH) containing macromolecules have been shown to be promising NO carriers. In the present study, we aimed to synthesize and characterize a potential NO carrier based on bovine Cu,Zn-superoxide dismutase (bSOD). To prepare S-nitrosated bSOD, the protein was incubated with S-nitrosoglutathione (GSNO) under varied experimental conditions. The results show that significant S-nitrosation of bSOD occurred only at high temperature (50 °C) for prolonged incubation time (>2 h) S-nitrosation efficiency increased with reaction time and reached a plateau at ~4 h. The maximum amo

SUBMITTER: Chen SH 

PROVIDER: S-EPMC3509561 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature