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Development of new polymorphic microsatellite loci for the barley stem gall midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an enriched library.


ABSTRACT: Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.

SUBMITTER: Mezghani-Khemakhem M 

PROVIDER: S-EPMC3509590 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Development of new polymorphic microsatellite loci for the barley stem gall midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an enriched library.

Mezghani-Khemakhem Maha M   Bouktila Dhia D   Casse Nathalie N   Maaroufi Houcine H   Makni Mohamed M   Makni Hanem H  

International journal of molecular sciences 20121108 11


Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population  ...[more]

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