Establishing epithelial glandular polarity: interlinked roles for ARF6, Rac1, and the matrix microenvironment.

ABSTRACT: Epithelial cysts comprise the structural units of the glandular epithelium. Although glandular inversion in epithelial tumors is thought to be a potential mechanism for the establishment of metastatic disease, little is known about the morphogenic cues and signaling pathways that govern glandular polarity and organization. Using organotypic cultures of Madin-Darby canine kidney cells in reconstituted basement membrane, we show that cellular depletion of the small GTP-binding protein ARF6 promotes the formation of inverted cysts, wherein the apical cell membrane faces the cyst exterior, and the basal domain faces the central lumen, while individual cell polarity is maintained. These cysts are also defective in interactions with laminin at the cyst-matrix interface. This inversion of glandul

SUBMITTER: Monteleon CL 

PROVIDER: S-EPMC3510012 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature