Human serum promotes osteogenic differentiation of human dental pulp stem cells in vitro and in vivo.

ABSTRACT: Human dental pulp is a promising alternative source of stem cells for cell-based tissue engineering in regenerative medicine, for the easily recruitment with low invasivity for the patient and for the self-renewal and differentiation potential of cells. So far, in vitro culture of mesenchymal stem cells is usually based on supplementing culture and differentiation media with foetal calf serum (FCS). FCS is known to contain a great quantity of growth factors, and thus to promote cell attachment on plastic surface as well as expansion and differentiation. Nevertheless, FCS as an animal origin supplement may represent a potential means for disease transmission besides leading to a xenogenic immune response. Therefore, a significant interest is focused on investigating alternative supplements,

SUBMITTER: Pisciotta A 

PROVIDER: S-EPMC3510089 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature