Suppression subtractive hybridization reveals transcript profiling of Chlorella under heterotrophy to photoautotrophy transition.

ABSTRACT:

Background

Microalgae have been extensively investigated and exploited because of their competitive nutritive bioproducts and biofuel production ability. Chlorella are green algae that can grow well heterotrophically and photoautotrophically. Previous studies proved that shifting from heterotrophy to photoautotrophy in light-induced environments causes photooxidative damage as well as distinct physiologic features that lead to dynamic changes in Chlorella intracellular components, which have great potential in algal health food and biofuel production. However, the molecular mechanisms underlying the trophic transition remain unclear.

Methodology/principal findings

In this study, suppression subtractive hybridization strategy was employed to screen and characterize genes that

SUBMITTER: Fan J 

PROVIDER: S-EPMC3510161 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature