Detection of dengue virus 4 from central India.

ABSTRACT:

Background & objectives

Dengue is an important arboviral disease. All four dengue virus serotypes are reported to be circulating in India. It is also known that different serotypes, genotypes and clades of genotype determine outbreak severity. Dengue affected children are known to have serious disease outcome. We carried out this study to give reliable diagnosis of dengue infection in children and to detect circulating serotype in central India.

Methods

Samples collected from paediatric patients suspected to have dengue fever were subjected to IgM and IgG ELISA to determine dengue virus infection. Samples collected within 0-5 days of onset of illness and positive by IgM ELISA were tested by nested reverse transcription polymerase chain reaction (nRT-PCR). The PCR products we

SUBMITTER: Barde PV 

PROVIDER: S-EPMC3510898 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature