Mannose-decorated cyclodextrin vesicles: The interplay of multivalency and surface density in lectin-carbohydrate recognition.

ABSTRACT: Cyclodextrin vesicles are versatile models for biological cell membranes since they provide a bilayer membrane that can easily be modified by host-guest interactions with functional guest molecules. In this article, we investigate the multivalent interaction of the lectin concanavalin A (ConA) with cyclodextrin vesicles decorated with mannose-adamantane conjugates with one, two or three adamantane units as well as one or two mannose units. The carbohydrate-lectin interaction in this artificial, self-assembled glycocalyx was monitored in an agglutination assay by the increase of optical density at 400 nm. It was found that there is a close relation between the carbohydrate density at the cyclodextrin vesicle surface and the multivalent interaction with ConA, and the most efficient interacti

SUBMITTER: Kauscher U 

PROVIDER: S-EPMC3510984 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature