RNA-seq pinpoints a Xanthomonas TAL-effector activated resistance gene in a large-crop genome.

ABSTRACT: Transcription activator-like effector (TALE) proteins of the plant pathogenic bacterial genus Xanthomonas bind to and transcriptionally activate host susceptibility genes, promoting disease. Plant immune systems have taken advantage of this mechanism by evolving TALE binding sites upstream of resistance (R) genes. For example, the pepper Bs3 and rice Xa27 genes are hypersensitive reaction plant R genes that are transcriptionally activated by corresponding TALEs. Both R genes have a hallmark expression pattern in which their transcripts are detectable only in the presence and not the absence of the corresponding TALE. By transcriptome profiling using next-generation sequencing (RNA-seq), we tested whether we could avoid laborious positional cloning for the isolation of TALE-induced R genes.

SUBMITTER: Strauss T 

PROVIDER: S-EPMC3511116 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature