A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli.

ABSTRACT:

Background

The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H:gm. The SefA protein was successfully exposed to the medium, but the orientation of H:gm in the outer membrane could not be determined due to proteolytic cleavage of the N-terminal detection-tag. The goal of the present work was therefore to construct a vector containing elements that facilitates analysis of surface expression, especially for proteins that are sensitive to proteolysis or otherwise difficult to express.

Results

The surface expression system pAIDA1 was created w

SUBMITTER: Jarmander J 

PROVIDER: S-EPMC3511212 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature