Co-expression analysis identifies putative targets for CBP60g and SARD1 regulation.

ABSTRACT:

Background

Salicylic acid is a critical signalling component in plant defence responses. In Arabidopsis, isochorismate synthase encoded by SID2 is essential for the biosynthesis of salicylic acid in response to biotic challenges. Recently, both the calmodulin binding protein CBP60g and its closest homolog, the non-calmodulin binding SARD1, have been shown to bind to the promoter region of SID2. Loss of both CBP60g and SARD1 severely impacts the plants ability to produce SA in response to bacterial inoculation and renders the plant susceptible to infection. In an electrophoretic mobility shift assay CBP60g and SARD1 were shown to bind specifically to a 10mer oligonucleotide with the sequence GAAATTTTGG.

Results

Gene expression profiling on a custom microarray identified a set

SUBMITTER: Truman W 

PROVIDER: S-EPMC3511238 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature