Differential capacity of human skin dendritic cells to polarize CD4+ T cells into IL-17, IL-21 and IL-22 producing cells.

ABSTRACT: Accumulating evidence suggests a contribution of T cell-derived IL-17, IL-21 and IL-22 cytokines in skin immune homeostasis as well as inflammatory disorders. Here, we analyzed whether the cytokine-producing T lymphocytes could be induced by the different subsets of human skin dendritic cells (DCs), i.e., epidermal Langerhans cells (LCs), dermal CD1c(+)CD14(-) and CD14(+) DCs (DDCs). DCs were purified following a 2-day migration from separated epidermal and dermal sheets and co-cultured with allogeneic T cells before cytokine secretion was explored. Results showed that no skin DCs could induce substantial IL-17 production by naïve CD4(+) or CD8(+)T lymphocytes whereas all of them could induce IL-17 production by memory T cells. In contrast, LCs and CD1c(+)CD14(-)DDCs were able to different

SUBMITTER: Penel-Sotirakis K 

PROVIDER: S-EPMC3511471 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature