Array-based profiling of DNA methylation changes associated with alcohol dependence.

ABSTRACT:

Background

Epigenetic regulation through DNA methylation may influence vulnerability to numerous disorders, including alcohol dependence (AD).

Methods

Peripheral blood DNA methylation levels of 384 CpGs in the promoter regions of 82 candidate genes were examined in 285 African Americans (AAs; 141 AD cases and 144 controls) and 249 European Americans (EAs; 144 AD cases and 105 controls) using Illumina GoldenGate Methylation Array assays. Association of AD and DNA methylation changes was analyzed using multivariate analyses of covariance with frequency of intoxication, sex, age, and ancestry proportion as covariates. CpGs showing significant methylation alterations in AD cases were further examined in a replication sample (49 EA cases and 32 EA controls) using Sequenom's MassA

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3511647 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature