In vivo two-photon microscopy reveals immediate microglial reaction to implantation of microelectrode through extension of processes.

ABSTRACT:

Objective

Penetrating cortical neural probe technologies allow investigators to record electrical signals in the brain. Implantation of probes results in acute tissue damage, and microglia density increases around implanted devices over weeks. However, the mechanisms underlying this encapsulation are not well understood in the acute temporal domain. The objective here was to evaluate dynamic microglial response to implanted probes using two-photon microscopy.

Approach

Using two-photon in vivo microscopy, cortical microglia ∼200 µm below the surface of the visual cortex were imaged every minute in mice with green fluorescent protein-expressing microglia.

Main results

Following probe insertion, nearby microglia immediately extended processes toward the probe at (1.6 ± 1

SUBMITTER: Kozai TD 

PROVIDER: S-EPMC3511663 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature