Force-clamp analysis techniques give highest rank to stretched exponential unfolding kinetics in ubiquitin.

ABSTRACT: Force-clamp spectroscopy reveals the unfolding and disulfide bond rupture times of single protein molecules as a function of the stretching force, point mutations, and solvent conditions. The statistics of these times reveal whether the protein domains are independent of one another, the mechanical hierarchy in the polyprotein chain, and the functional form of the probability distribution from which they originate. It is therefore important to use robust statistical tests to decipher the correct theoretical model underlying the process. Here, we develop multiple techniques to compare the well-established experimental data set on ubiquitin with existing theoretical models as a case study. We show that robustness against filtering, agreement with a maximum likelihood function that takes into

SUBMITTER: Lannon H 

PROVIDER: S-EPMC3512049 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature