Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus.

ABSTRACT:

Background

The rising demand for pork has resulted in a massive expansion of pig production in Uganda. This has resulted in increased contact between humans and pigs. Pigs can act as reservoirs for emerging infectious diseases. Therefore identification of potential zoonotic pathogens is important for public health surveillance. In this study, during a routine general surveillance for African swine fever, domestic pigs from Uganda were screened for the presence of RNA and DNA viruses using a high-throughput pyrosequencing method.

Findings

Serum samples from 16 domestic pigs were collected from five regions in Uganda and pooled accordingly. Genomic DNA and RNA were extracted and sequenced on the 454 GS-FLX platform. Among the sequences assigned to a taxon, 53% mapped to the do

SUBMITTER: Masembe C 

PROVIDER: S-EPMC3512490 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature