Orai1 and CRAC channel dependence of VEGF-activated Ca2+ entry and endothelial tube formation.

ABSTRACT:

Rationale

Orai1 and the associated calcium release-activated calcium (CRAC) channel were discovered in the immune system. Existence also in endothelial cells has been suggested, but the relevance to endothelial biology is mostly unknown.

Objective

The aim of this study was to investigate the relevance of Orai1 and CRAC channels to vascular endothelial growth factor (VEGF) and endothelial tube formation.

Methods and results

In human umbilical vein endothelial cells, Orai1 disruption by short-interfering RNA or dominant-negative mutant Orai1 inhibited calcium release-activated (store-operated) calcium entry, VEGF-evoked calcium entry, cell migration, and in vitro tube formation. Expression of exogenous wild-type Orai1 rescued the tube formation. VEGF receptor-2 and Orai

SUBMITTER: Li J 

PROVIDER: S-EPMC3512576 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature