93 Purification of the Allergenic Protein Hyaluronidase From the Venom of Social Wasp Polybia Paulista (Hymenoptera: Vespidae)

ABSTRACT:

Background

Between 9.3 and 28.5% of world population are estimated to be allergic to the protein components of Hymenoptera venom and among them, the hyaluronidase (HYAL) is one of the most important, although the nature of its IgE binding epitopes is unknown.

Methods

Enzyme was purified from P. paulista venom by cation exchange chromatography in AKTA-FPLC system with a Hiprep CM FF column. Fractions with HYAL activity were pooled, lyophilized, dialyzed against distilled water in presence of protease inhibitor (PMSF) and subjected to SDS-PAGE 15%. The only protein bands visualized were submitted to tryptic digestion and to MALDI-ToF/ToF mass spectrometry. Digested peptides produced were compared with partial/complete protein sequences from Data Bank including with the HYAL

SUBMITTER: Justo Jacomini D 

PROVIDER: S-EPMC3512878 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature