Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis.

ABSTRACT:

Background

An in vitro artificial feeding technique for hard ticks is quite useful for studying the tick-pathogen interactions. Here, we report a novel semi-artificial feeding technique for the adult parthenogenetic tick, Haemaphysalis longicornis, using mouse skin membrane.

Findings

Skin with attached adult ticks was removed from the mouse body at 4 to 5 days post-infestation for the construction of the feeding system. This system supplied with rabbit blood was kept in >95% relative humidity at 30°C during the feeding, and ticks were fully engorged (artificially engorged, AE) within 12 to 48 h. For comparison, ticks were fed to engorgement solely on rabbit or mouse for 5 days as controls (naturally engorged on rabbit, NEr, or mouse, NEm). Blood digestion-related gene expres

SUBMITTER: Hatta T 

PROVIDER: S-EPMC3514109 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature