Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.

ABSTRACT:

Background

Interferon-induced 35-kDa protein (IFP35) plays important roles in antiviral defense and the progression of some skin cancer diseases. It can be induced by interferon-γ (IFN-γ) in multiple human cells. However, the mechanisms by which IFN-γ contributes to IFP35 induction remain to be elucidated.

Methods/principal findings

We identified the transcription start sites of IFP35 by 5' rapid amplification of cDNA ends (RACE) and cloned the promoter of IFP35. Sequence analysis and luciferase assays revealed two GC boxes and an IFN-stimulated response element (ISRE) in the 5' upstream region of the transcription start sites, which were important for the basal transcription of IFP35 gene. Furthermore, we found that interferon regulatory factor 1 (IRF-1) and IRF-2 could bin

SUBMITTER: Yang W 

PROVIDER: S-EPMC3514179 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature