Statistical adjustment of genotyping error in a case-control study of childhood leukaemia.

ABSTRACT:

Background

Genotyping has become more cost-effective and less invasive with the use of buccal cell sampling. However, low or fragmented DNA yields from buccal cells collected using FTA cards often requires additional whole genome amplification to produce sufficient DNA for genotyping. In our case-control study of childhood leukaemia, discordance was found between genotypes derived from blood and whole genome amplified FTA buccal DNA samples. We aimed to develop a user-friendly method to correct for this genotype misclassification, as existing methods were not suitable for use in our study.

Methods

Discordance between the results of blood and buccal-derived DNA was assessed in childhood leukaemia cases who had both blood and FTA buccal samples. A method based on applying misc

SUBMITTER: Cooper MN 

PROVIDER: S-EPMC3514307 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature