The binning of metagenomic contigs for microbial physiology of mixed cultures.

ABSTRACT: So far, microbial physiology has dedicated itself mainly to pure cultures. In nature, cross feeding and competition are important aspects of microbial physiology and these can only be addressed by studying complete communities such as enrichment cultures. Metagenomic sequencing is a powerful tool to characterize such mixed cultures. In the analysis of metagenomic data, well established algorithms exist for the assembly of short reads into contigs and for the annotation of predicted genes. However, the binning of the assembled contigs or unassembled reads is still a major bottleneck and required to understand how the overall metabolism is partitioned over different community members. Binning consists of the clustering of contigs or reads that apparently originate from the same source popula

SUBMITTER: Strous M 

PROVIDER: S-EPMC3514610 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature