A fine scale phenotype-genotype virulence map of a bacterial pathogen.

ABSTRACT: A large fraction of the genes from sequenced organisms are of unknown function. This limits biological insight, and for pathogenic microorganisms hampers the development of new approaches to battle infections. There is thus a great need for novel strategies that link genotypes to phenotypes for microorganisms. We describe a high-throughput strategy based on the method Tn-seq that can be applied to any genetically manipulatable microorganism. By screening 17 in vitro and two in vivo (carriage and infection) conditions for the pathogen Streptococcus pneumoniae, we create a resource consisting of >1800 interactions that is rich in new genotype-phenotype relationships. We describe genes that are involved in differential carbon source utilization in the host, as well as genes that are involved

SUBMITTER: van Opijnen T 

PROVIDER: S-EPMC3514683 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature