Functional monoclonal antibody acts as a biased agonist by inducing internalization of metabotropic glutamate receptor 7.

ABSTRACT:

Background and purpose

The mGlu(7) receptors are strategically located at the site of vesicle fusion where they modulate the release of the main excitatory and inhibitory neurotransmitters. Consequently, they are implicated in the underlying pathophysiology of CNS diseases such as epilepsy and stress-related psychiatric disorders. Here, we characterized a selective, potent and functional anti-mGlu(7) monoclonal antibody, MAB1/28, that triggers receptor internalization.

Experimental approach

MAB1/28's activity was investigated using Western blot and direct immunofluorescence on live cells, in vitro pharmacology by functional cAMP and [(35) S]-GTPγ binding assays, the kinetics of IgG-induced internalization by image analysis, and the activation of the ERK1/2 by elisa.

Key r

SUBMITTER: Ullmer C 

PROVIDER: S-EPMC3514759 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature