Association analysis between SNPs in IL-28B gene and the progress of hepatitis B infection in Han Chinese.

ABSTRACT:

Objective

As a candidate gene association study, we investigated the genetic association of SNPs in IL-28B genes with different outcomes of HBV infection, including LC and HCC occurrence.

Methods

Chinese Han subjects were categorized into two groups: 406 LC caused by CHB and 406 HCC caused by CHB. Genomic DNA was isolated from whole blood samples, SNPs were detected using high resolution melting curve (HRM) method. PCR amplification was carried out under the same conditions in a 96-well plate in Real-Time PCR System. Then 341 LC and 356 HCC patients caused by HBV infection were analyzed as a verification by independent sample. 393 CHB patients and 244 health subjects were included as control.

Results

CHB patients who progress to LC or HCC showed a significant differen

SUBMITTER: Chen J 

PROVIDER: S-EPMC3515436 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature