Cadherin point mutations alter cell sorting and modulate GTPase signaling.

ABSTRACT: This study investigated the impact of cadherin binding differences on both cell sorting and GTPase activation. The use of N-terminal domain point mutants of Xenopus C-cadherin enabled us to quantify binding differences and determine their effects on cadherin-dependent functions without any potential complications arising as a result of differences in cytodomain interactions. Dynamic cell-cell binding measurements carried out with the micropipette manipulation technique quantified the impact of these mutations on the two-dimensional binding affinities and dissociation rates of cadherins in the native context of the cell membrane. Pairwise binding affinities were compared with in vitro cell-sorting specificity and ligation-dependent GTPase signaling. Two-dimensional affinity differences grea

SUBMITTER: Tabdili H 

PROVIDER: S-EPMC3516376 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature