Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transposition.

ABSTRACT: Heightened interest in relevant models for human disease increases the need for improved methods for germline transgenesis. We describe a significant improvement in the creation of transgenic laboratory mice and rats by chemical modification of Sleeping Beauty transposons. Germline transgenesis in mice and rats was significantly enhanced by in vitro cytosine-phosphodiester-guanine methylation of transposons prior to injection. Heritability of transgene alleles was also greater from founder mice generated with methylated versus non-methylated transposon. The artificial methylation was reprogrammed in the early embryo, leading to founders that express the transgenes. We also noted differences in transgene insertion number and structure (single-insert versus concatemer) based on the influence

SUBMITTER: Carlson DF 

PROVIDER: S-EPMC3516389 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature