Sonic hedgehog regulates presynaptic terminal size, ultrastructure and function in hippocampal neurons.

ABSTRACT: Sonic hedgehog (Shh) signaling is essential to the patterning of the embryonic neural tube, but its presence and function in the postmitotic differentiated neurons in the brain remain largely uncharacterized. We recently showed that Shh and its signaling components, Patched and Smoothened, are expressed in postnatal and adult hippocampal neurons. We have now examined whether Shh signaling has a function in these neurons. Using cultured hippocampal neurons as a model system, we found that presynaptic terminals become significantly larger in response to the application of Shh. Ultrastructural examination confirmed the enlarged presynaptic profiles and also revealed variable increases in the size of synaptic vesicles, with a resulting loss of uniformity. Furthermore, electrophysiological anal

SUBMITTER: Mitchell N 

PROVIDER: S-EPMC3516435 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature