Pyrosequencing of Haliotis diversicolor transcriptomes: insights into early developmental molluscan gene expression.

ABSTRACT:

Background

The abalone Haliotis diversicolor is a good model for study of the settlement and metamorphosis, which are widespread marine ecological phenomena. However, information on the global gene backgrounds and gene expression profiles for the early development of abalones is lacking.

Methodology/principal findings

In this study, eight non-normalized and multiplex barcode-labeled transcriptomes were sequenced using a 454 GS system to cover the early developmental stages of the abalone H. diversicolor. The assembly generated 35,415 unigenes, of which 7,566 were assigned GO terms. A global gene expression profile containing 636 scaffolds/contigs was constructed and was proven reliable using qPCR evaluation. It indicated that there may be existing dramatic phase transitions.

SUBMITTER: Huang ZX 

PROVIDER: S-EPMC3517415 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature