The peptide derived from the Ig-like domain of human herpesvirus 8 K1 protein induces death in hematological cancer cells.

ABSTRACT:

Background

Although significant progress has been made in the treatment of lymphomas, many lymphomas exhibit resistance to cell death, suggesting a defective Fas signaling, which remains poorly understood. We previously reported that cells expressing the K1 protein of human herpesvirus 8 (HHV-8) resist death through the complex formation of the Ig-like domain of K1 with Fas. Recently, we investigated whether peptides derived from the Ig-like domain of the K1 protein may affect cell death.

Methods

K1 positive and negative cell lines were incubated with the K1-derived peptides, and cell death (apoptotic and necrotic) was assessed by flow cytometry and LDH assay. Activation of caspases was assessed by fluorometric assay and flow cytometry. Fas receptor-independent, peptide-medi

SUBMITTER: Daniluk U 

PROVIDER: S-EPMC3517441 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature