In vitro and in vivo development of mice morulae after storage in non-frozen conditions.

ABSTRACT:

Background

Interchange of genetically modified (GM) mice between laboratories using embryos provides several advantages. Not only is transport stress avoided, but also the health status of the recipient colony is not compromised. Embryos do not need to be shipped in frozen stage, which requires expensive packaging in addition to a certain degree of expertise in order to freeze and thaw them correctly. The aim of this study was to examine different storage conditions and their effect on embryo viability in order to establish the feasibility of practical, non-frozen conditions for embryo shipment.

Methods

Mouse morulae developed in vivo (collected from donors 2.5d post coitum) or in vitro (zygotes cultured until morulae stage) were stored, combining two different media (KSOMeq

SUBMITTER: de Dios Hourcade J 

PROVIDER: S-EPMC3517773 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature