Structural and quantitative evidence for dynamic glycome shift on production of induced pluripotent stem cells.

ABSTRACT: We recently reported that induced pluripotent stem cells (iPSCs) prepared from different human origins acquired similar glycan profiles to one another as well as to human embryonic stem cells. Although the results strongly suggested attainment of specific glycan expressions associated with the acquisition of pluripotency, the detailed glycan structures remained to be elucidated. Here, we perform a quantitative glycome analysis targeting both N- and O-linked glycans derived from 201B7 human iPSCs and human dermal fibroblasts as undifferentiated and differentiated cells, respectively. Overall, the fractions of high mannose-type N-linked glycans were significantly increased upon induction of pluripotency. Moreover, it became evident that the type of linkage of Sia on N-linked glycans was dram

SUBMITTER: Hasehira K 

PROVIDER: S-EPMC3518133 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature