High-definition de novo sequencing of crustacean hyperglycemic hormone (CHH)-family neuropeptides.

ABSTRACT: A complete understanding of the biological functions of large signaling peptides (>4 kDa) requires comprehensive characterization of their amino acid sequences and post-translational modifications, which presents significant analytical challenges. In the past decade, there has been great success with mass spectrometry-based de novo sequencing of small neuropeptides. However, these approaches are less applicable to larger neuropeptides because of the inefficient fragmentation of peptides larger than 4 kDa and their lower endogenous abundance. The conventional proteomics approach focuses on large-scale determination of protein identities via database searching, lacking the ability for in-depth elucidation of individual amino acid residues. Here, we present a multifaceted MS approach for iden

SUBMITTER: Jia C 

PROVIDER: S-EPMC3518134 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature