RNA-Seq vs dual- and single-channel microarray data: sensitivity analysis for differential expression and clustering.

ABSTRACT: With the fast development of high-throughput sequencing technologies, a new generation of genome-wide gene expression measurements is under way. This is based on mRNA sequencing (RNA-seq), which complements the already mature technology of microarrays, and is expected to overcome some of the latter's disadvantages. These RNA-seq data pose new challenges, however, as strengths and weaknesses have yet to be fully identified. Ideally, Next (or Second) Generation Sequencing measures can be integrated for more comprehensive gene expression investigation to facilitate analysis of whole regulatory networks. At present, however, the nature of these data is not very well understood. In this paper we study three alternative gene expression time series datasets for the Drosophila melanogaster embryo

SUBMITTER: Sirbu A 

PROVIDER: S-EPMC3518479 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature