She1-mediated inhibition of dynein motility along astral microtubules promotes polarized spindle movements.

ABSTRACT:

Background

Cytoplasmic dynein motility along microtubules is critical for diverse cellular processes ranging from vesicular transport to nuclear envelope breakdown to mitotic spindle alignment. In yeast, we have proposed a regulated-offloading model to explain how dynein motility drives microtubule sliding along the cortex, powering transport of the nucleus into the mother-bud neck [1, 2]: the dynein regulator She1 limits dynein offloading by gating the recruitment of dynactin to the astral microtubule plus end, a prerequisite for offloading to the cortex. However, whether She1 subsequently affects cortically anchored dynein activity during microtubule sliding is unclear.

Results

Using single-molecule motility assays, we show that She1 strongly inhibits dynein movement along

SUBMITTER: Markus SM 

PROVIDER: S-EPMC3518626 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature