External push and internal pull forces recruit curvature-sensing N-BAR domain proteins to the plasma membrane.

ABSTRACT: Many of the more than 20 mammalian proteins with N-BAR domains control cell architecture and endocytosis by associating with curved sections of the plasma membrane. It is not well understood whether N-BAR proteins are recruited directly by processes that mechanically curve the plasma membrane or indirectly by plasma-membrane-associated adaptor proteins that recruit proteins with N-BAR domains that then induce membrane curvature. Here, we show that externally induced inward deformation of the plasma membrane by cone-shaped nanostructures (nanocones) and internally induced inward deformation by contracting actin cables both trigger recruitment of isolated N-BAR domains to the curved plasma membrane. Markedly, live-cell imaging in adherent cells showed selective recruitment of full-length N-B

SUBMITTER: Galic M 

PROVIDER: S-EPMC3519285 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature