FOLD-EM: automated fold recognition in medium- and low-resolution (4-15 A) electron density maps.

ABSTRACT:

Motivation

Owing to the size and complexity of large multi-component biological assemblies, the most tractable approach to determining their atomic structure is often to fit high-resolution radiographic or nuclear magnetic resonance structures of isolated components into lower resolution electron density maps of the larger assembly obtained using cryo-electron microscopy (cryo-EM). This hybrid approach to structure determination requires that an atomic resolution structure of each component, or a suitable homolog, is available. If neither is available, then the amount of structural information regarding that component is limited by the resolution of the cryo-EM map. However, even if a suitable homolog cannot be identified using sequence analysis, a search for structural homologs sh

SUBMITTER: Saha M 

PROVIDER: S-EPMC3519459 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature