A method for integrative structure determination of protein-protein complexes.

ABSTRACT:

Motivation

Structural characterization of protein interactions is necessary for understanding and modulating biological processes. On one hand, X-ray crystallography or NMR spectroscopy provide atomic resolution structures but the data collection process is typically long and the success rate is low. On the other hand, computational methods for modeling assembly structures from individual components frequently suffer from high false-positive rate, rarely resulting in a unique solution.

Results

Here, we present a combined approach that computationally integrates data from a variety of fast and accessible experimental techniques for rapid and accurate structure determination of protein-protein complexes. The integrative method uses atomistic models of two interacting proteins

SUBMITTER: Schneidman-Duhovny D 

PROVIDER: S-EPMC3519461 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature