TMEM45A is essential for hypoxia-induced chemoresistance in breast and liver cancer cells.

ABSTRACT:

Background

Hypoxia is a common characteristic of solid tumors associated with reduced response to radio- and chemotherapy, therefore increasing the probability of tumor recurrence. The aim of this study was to identify new mechanisms responsible for hypoxia-induced resistance in breast cancer cells.

Methods

MDA-MB-231 and HepG2 cells were incubated in the presence of taxol or etoposide respectively under normoxia and hypoxia and apoptosis was analysed. A whole transcriptome analysis was performed in order to identify genes whose expression profile was correlated with apoptosis. The effect of gene invalidation using siRNA was studied on drug-induced apoptosis.

Results

MDA-MB-231 cells incubated in the presence of taxol were protected from apoptosis and cell death by hy

SUBMITTER: Flamant L 

PROVIDER: S-EPMC3519606 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature