Identification of suitable reference genes for real-time PCR analysis of statin-treated human umbilical vein endothelial cells.

ABSTRACT: Proper data normalization in quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) is of critical importance for reliable mRNA expression analysis. Due to a diversity in putative reference genes expression stability in different in vitro models, a validation of an internal control gene should be made for each particular tissue or cell type and every specific experimental design. A few approaches have been proposed for reference gene selection, including pair-wise comparison approach and model-based approach. In this article we have assessed the expression stability of eight putative reference genes: ACTB, B2M, GADD45A, GAPDH, HPRT1, PES1, PSMC4, YWHAZ, in human umbilical vein endothelial cells (HUVEC) treated with different statins and with TNF-α. The analysis wa

SUBMITTER: Zyzynska-Granica B 

PROVIDER: S-EPMC3519707 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature