Variation in dengue virus plaque reduction neutralization testing: systematic review and pooled analysis.

ABSTRACT:

Background

The plaque reduction neutralization test (PRNT) remains the gold standard for the detection of serologic immune responses to dengue virus (DENV). While the basic concept of the PRNT remains constant, this test has evolved in multiple laboratories, introducing variation in materials and methods. Despite the importance of laboratory-to-laboratory comparability in DENV vaccine development, the effects of differing PRNT techniques on assay results, particularly the use of different dengue strains within a serotype, have not been fully characterized.

Methods

We conducted a systematic review and pooled analysis of published literature reporting individual-level PRNT titers to identify factors associated with heterogeneity in PRNT results and compared variation between s

SUBMITTER: Rainwater-Lovett K 

PROVIDER: S-EPMC3519720 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature