Multivalent display of the antimicrobial peptides BP100 and BP143.

ABSTRACT: Carbohydrates are considered as promising templates for the display of multiple copies of antimicrobial peptides. Herein, we describe the design and synthesis of chimeric structures containing two or four copies of the antimicrobial peptides KKLFKKILKYL-NH(2) (BP100) and KKLfKKILKYL-NH(2) (BP143) attached to the carbohydrate template cyclodithioerythritol (cDTE) or α-D-galactopyranoside (Galp). The synthesis involved the preparation of the corresponding peptide aldehyde followed by coupling to an aminooxy-functionalized carbohydrate template. After purification, the multivalent display systems were obtained in high purities (90-98%) and in good yields (42-64%). These compounds were tested against plant and human pathogenic bacteria and screened for their cytotoxicity on eukaryotic cells. T

SUBMITTER: Guell I 

PROVIDER: S-EPMC3520567 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature