Regulation of ROCK1 via Notch1 during breast cancer cell migration into dense matrices.

ABSTRACT:

Background

The behaviour of tumour cells depends on factors such as genetics and the tumour microenvironment. The latter plays a crucial role in normal mammary gland development and also in breast cancer initiation and progression. Breast cancer tissues tend to be highly desmoplastic and dense matrix as a pre-existing condition poses one of the highest risk factors for cancer development. However, matrix influence on tumour cell gene expression and behaviour such as cell migration is not fully elucidated.

Results

We generated high-density (HD) matrices that mimicked tumour collagen content of 20 mg/cm3 that were ~14-fold stiffer than low-density (LD) matrix of 1 mg/cm3. Live-cell imaging showed breast cancer cells utilizing cytoplasmic streaming and cell body contractility f

SUBMITTER: Raviraj V 

PROVIDER: S-EPMC3520698 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature