Rapid production of antigen-specific monoclonal antibodies from a variety of animals.

ABSTRACT:

Background

Although a variety of animals have been used to produce polyclonal antibodies against antigens, the production of antigen-specific monoclonal antibodies from animals remains challenging.

Results

We propose a simple and rapid strategy to produce monoclonal antibodies from a variety of animals. By staining lymph node cells with an antibody against immunoglobulin and a fluorescent dye specific for the endoplasmic reticulum, plasma/plasmablast cells were identified without using a series of antibodies against lineage markers. By using a fluorescently labeled antigen as a tag for a complementary cell surface immunoglobulin, antigen-specific plasma/plasmablast cells were sorted from the rest of the cell population by fluorescence-activated cell sorting. Amplification of

SUBMITTER: Kurosawa N 

PROVIDER: S-EPMC3520816 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature