Human regulatory T cells of G-CSF mobilized allogeneic stem cell donors qualify for clinical application.

ABSTRACT: Recent clinical studies demonstrate the high potency of regulatory T cells (Tregs) to control graft-versus-host disease in hematopoietic stem cell transplantation (SCT). However, the adoptive transfer of Tregs is limited by their low frequency in unstimulated donors and considerable concerns that G-CSF induced SC mobilization might have negative effects on the stability and function of Tregs. The isolation of Tregs from the G-CSF mobilized SC grafts would extend this novel strategy for tolerance induction to the unrelated setting and simplify global clinical application. We characterized CD4(+)CD25(high)CD127(-) Tregs from SC donors before and after G-CSF mobilization for their phenotype, function, and stability. After G-CSF application the Treg cell yield increased significantly. Donor Tr

SUBMITTER: Ukena SN 

PROVIDER: S-EPMC3520921 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature