Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants.

ABSTRACT: Nickel-containing hydrogenases, the biological catalysts of oxidation and production, reversibly inactivate under anaerobic, oxidizing conditions. We aim at understanding the mechanism of (in)activation and what determines its kinetics, because there is a correlation between fast reductive reactivation and oxygen tolerance, a property of some hydrogenases that is very desirable from the point of view of biotechnology. Direct electrochemistry is potentially very useful for learning about the redox-dependent conversions between active and inactive forms of hydrogenase, but the voltammetric signals are complex and often misread. Here we describe simple analytical models that we used to characterize and compare 16 mutants, obtained by substituting the position-74 valine of the -sensitiv

SUBMITTER: Abou Hamdan A 

PROVIDER: S-EPMC3523816 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature