Identification and expression of nine oak aquaporin genes in the primary root axis of two oak species, Quercus petraea and Quercus robur.

ABSTRACT: Aquaporins (AQPs) belong to the Major Intrinsic Protein family that conducts water and other small solutes across biological membranes. This study aimed to identify and characterize AQP genes in the primary root axis of two oak species, Quercus petraea and Quercus robur. Nine putative AQP genes were cloned, and their expression was profiled in different developmental root zones by real-time PCR. A detailed examination of the predicted amino acid sequences and subsequent phylogenetic analysis showed that the isolated AQPs could be divided into two subfamilies, which included six plasma membrane intrinsic proteins (PIPs) and three tonoplast intrinsic proteins (TIPs). We characterized the anatomical features of the roots and defined three developmental root zones: the immature, transition and

SUBMITTER: Rasheed-Depardieu C 

PROVIDER: S-EPMC3524086 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature