Spatial and temporal gene expression differences in core and periinfarct areas in experimental stroke: a microarray analysis.

ABSTRACT:

Background

A large number of genes are regulated to promote brain repair following stroke. The thorough analysis of this process can help identify new markers and develop therapeutic strategies. This study analyzes gene expression following experimental stroke.

Methodology/principal findings

A microarray study of gene expression in the core, periinfarct and contralateral cortex was performed in adult Sprague-Dawley rats (n = 60) after 24 hours (acute phase) or 3 days (delayed stage) of permanent middle cerebral artery (MCA) occlusion. Independent qRT-PCR validation (n = 12) was performed for 22 of the genes. Functional data were evaluated by Ingenuity Pathway Analysis. The number of genes differentially expressed was 2,612 (24 h) and 5,717 (3 d) in the core; and 3,505 (24 h)

SUBMITTER: Ramos-Cejudo J 

PROVIDER: S-EPMC3524135 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature