Retrospective analysis of the HIV-1 reverse transcriptase inhibitors' resistance in Silesia, Poland.

ABSTRACT:

Background

This study was a retrospective analysis of drug resistance mutations among HIV-1 strains prevalent in Silesia, Poland, from the origin of the epidemic to 2004. The investigations included both type and frequency of the reverse transcriptase inhibitors' resistance mutations and estimation of the drugs' resistance levels.

Material/methods

Proviral DNA, obtained from peripheral blood mononuclear cells of the 101 HIV-1-infected patients, was amplified and sequenced in the pol gene fragment covering the first 256 codons of the reverse transcriptase (RT). Reverse transcriptase inhibitors resistance mutations were determined and interpreted with the HIVdb: Genotypic Resistance Interpretation Algorithm available from the Stanford University HIV Drug Resistance Database. I

SUBMITTER: Smolen J 

PROVIDER: S-EPMC3524699 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature