Electrophoretic build-up of multi nanoparticle array for a highly sensitive immunoassay.

ABSTRACT: One of the challenges in shrinking immunoassays to smaller sizes is to immobilize the biological molecules to nanometer-scaled spots. To overcome this complication, we have employed a particle-based immunoassay to create a nanostructured platform with a regular array of sensing elements. The technique makes use of an electrophoretic particle entrapment system (EPES) to immobilize nanoparticles that are coated with biological reagents into wells using a very small trapping potential. To provide useful information for controlling the trapping force and optimal design of the nanoarray, electrophoretic trapping of a nanoparticle was modeled numerically. The trapping efficiency, defined as the fraction of wells occupied by a single particle, was 91%. The performance of the array was demonstrate

SUBMITTER: Han JH 

PROVIDER: S-EPMC3525773 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature